To correct errors associated with the superposition of the studied organisms in the frame projection and identify individual characteristics of animals, tracking is applied. For each organism with on the current frame, the vector of characteristics is calculated - (x,y,x1,y1,x2,y2), where (x,y) are the coordinates of the center of the organism, (x1, y1) are the projected velocities of the body on the plane of the frame in the corresponding axes, (x2, y2) - projections of accelerations on the plane of the frame in the corresponding axes. Further, on the next frame, similar vectors are calculated for all detected objects, after which a covariance matrix of Daphnia correspondence between frames is compiled. From which the movement of each specific object between frames is determined, as well as their merging/divergence.