Mutations in the chico, E(z), puc genes

The combination in one genome of mutations in several genes, for each of them separately earlier studies have shown an effect on lifespan.

Model organism: Drosophila melanogaster

Number of genes: 3

Type of combination: combination of mutations in selected genes

Difficulty of combination: normal

Gene chico and mutation chico[1]

Gene E(z) and mutation E(z)[731]

Gene puc and mutation puc[A251.1F3]

Gene chico and mutation chico[1]

Gene in the FlyBase database
Genomic location of the gene: 2L: 10,242,512..10,247,078 [-]
Involvement in the signaling pathways/processes: the insulin signaling pathway, nutrient sensitivity
Human ortholog: IRS1
Link to description of IRS1 in the database of genes associated with aging - Open Genes
Mutation name: 1
Link to description of the mutation in the FlyBase
Type of the mutation: P-element
Mutation effect on the gene: protein loss-of-function
Detailed description of the mutation: P{ry11} transposon insertion 80 bp downstream of the putative translation initiation site
The publication on the mutation
Publication on the impact of gene expression change on longevity
Mutation effect on longevity: ↑ 41-47%
Fly line containing this mutation: BDSC (Bloomington Drosophila Stock Center) #10738 cn[1]P{ry[+t7.2]=ry11}chico[1]

Gene in the FlyBase database
Genomic location of the gene: 3L: 10,634,575..10,638,130 [-]
Involvement in the signaling pathways/processes: epigenetics
Human ortholog: EZH2
Link to description of EZH2 in the database of genes associated with aging - Open Genes
Mutation name: 731
Link to description of the mutation in the FlyBase
Type of the mutation: EMS
Detailed description of the mutation: point mutation, substitution of guanine for adenine, which leads to a change in the amino acid sequence: W638term (TGG to TGA)
Publication on the impact of gene change on longevity
Mutation effect on longevity: ↑ 76%
Fly line containing this mutation: BDSC (Bloomington Drosophila Stock Center) #24470 E(z)[731] P{1xFRT.G}2A

Gene in the FlyBase database
Genomic location of the gene: 3R: coordinates 8,105,332..8,122,294 [+]
Involvement in the signaling pathways/processes: the JNK cascade (c-Jun N-terminal kinase)
Human ortholog: DUSP10
Link to description of DUSP10 in the database of genes associated with aging - Open Genes
Mutation name: A251.1F3
Link to description of the mutation in the FlyBase database
Type of the mutation: P-element
Note: the mutation is lethal in the homozygous state
Detailed description of the mutation: P{lArB} transposon insertion in the second intron of the puc gene
The publication on the mutation
Publication on the impact of gene change on longevity
Mutation effect on longevity: ↑ 38%
Fly line containing this mutation: BDSC (Bloomington Drosophila Stock Center) #11173 P{ry[+t7.2]=lArB}puc[A251.1F3]