Вack to combinations

Overexpression of two genes from: foxo, Lam, Gclc, Sirt4, MsrA

Targeted overexpression of genes is carried out using the dCas9-VPR system. It is possible to choose a combination of 2 genes from: foxo, Lam, Gclc, Sirt4, MsrA.

Model organism: Drosophila melanogaster

Number of genes: 2

Type of combination: targeted overexpression of genes by the dCas9-VPR system

Difficulty of combination: normal

Gene foxo
Gene Lam
Gene Gclc
Gene Sirt4
Gene MsrA

Gene foxo

Gene Lam

  • Gene in the Open Genes database
  • Publications of the impact of gene change on longevity
  • Involvement in the signaling pathways/processes: epigenetics
  • Effect of the gene expression change on lifespan: is reduced by 40% in case of knockdown in neurons
  • Human ortholog(s): LMNA, LMNB2, LMNB1
  • Fly line bearing the sgRNA transgene(s): BDSC (Bloomington Drosophila Stock Center) #78653
  • Genotype of the transgenic line: P{y[+t7.7] v[+t1.8]=TOE.GS01904}attP40
  • Transgene localization (chromosome): 2
  • Description of the transgene: ubiquitously expresses a single guide RNA for Lam overexpression by a Cas9-based transcriptional activator

Gene Gclc

   1) BDSC (Bloomington Drosophila Stock Center)  #68141
   2) BDSC (Bloomington Drosophila Stock Center)  #68142
  • Genotype of the transgenic line:
  1) P{y[+t7.7] v[+t1.8]=TOE.GS00538}attP40
  2) P{y[+t7.7] v[+t1.8]=TOE.GS00539}attP40
  • Transgene localization (chromosome): 2
  • Description of the transgene: ubiquitously expresses a single guide RNA for Gclc overexpression by a Cas9-based transcriptional activator

Gene Sirt4

  • Gene in the Open Genes database
  • Publications of the impact of gene change on longevity
  • Involvement in the signaling pathways/processes: mitochondrial function, oxidative metabolism
  • Effect of the gene expression change on lifespan: is reduced by 40% in case of Sirt4 knockout, increases by 20% in case of ubiquitous overexpression of Sirt4.
  • Human ortholog(s): SIRT4
  • Fly line bearing the sgRNA transgene(s): BDSC (Bloomington Drosophila Stock Center) #78741
  • Genotype of the transgenic line: P{y[+t7.7] v[+t1.8]=TOE.GS02264}attP40
  • Transgene localization (chromosome): 2
  • Description of the transgene: ubiquitously expresses a single guide RNA for Sirt4 overexpression by a Cas9-based transcriptional activator

Gene MsrA

  • Gene in the Open Genes database
  • Publications of the impact of gene change on longevity: FEBS Letters, scientific reports, PNAS
  • Involvement in the signaling pathways/processes: oxidative stress
  • Effect of the gene expression change on lifespan: is increased by 70-80% in case of overexpression of MsrA in neurons.
  • Human ortholog(s): MSRA
  • Fly line bearing the sgRNA transgene(s):  BDSC (Bloomington Drosophila Stock Center) #81388
  • Genotype of the transgenic line: P{y[+t7.7]v[+t1.8]=SAM.dCas9.GS03907}attP40
  • Transgene localization (chromosome): 2
  • Description of the transgene: it ubiquitously expresses a single guide RNA for Eip71CD (MsrA) overexpression. It also expresses the flySAM components, dCas9::VP64 and MCP::p65(h)::HSF1, under UAS control. The UAS:SAM construct was 100% lethal when ubiquitously expressed (using actin-Gal4)